258 research outputs found

    A Genome-Wide Analysis of Promoter-Mediated Phenotypic Noise in Escherichia coli

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    Gene expression is subject to random perturbations that lead to fluctuations in the rate of protein production. As a consequence, for any given protein, genetically identical organisms living in a constant environment will contain different amounts of that particular protein, resulting in different phenotypes. This phenomenon is known as “phenotypic noise.” In bacterial systems, previous studies have shown that, for specific genes, both transcriptional and translational processes affect phenotypic noise. Here, we focus on how the promoter regions of genes affect noise and ask whether levels of promoter-mediated noise are correlated with genes' functional attributes, using data for over 60% of all promoters in Escherichia coli. We find that essential genes and genes with a high degree of evolutionary conservation have promoters that confer low levels of noise. We also find that the level of noise cannot be attributed to the evolutionary time that different genes have spent in the genome of E. coli. In contrast to previous results in eukaryotes, we find no association between promoter-mediated noise and gene expression plasticity. These results are consistent with the hypothesis that, in bacteria, natural selection can act to reduce gene expression noise and that some of this noise is controlled through the sequence of the promoter region alon

    Image-guided ToF depth upsampling: a survey

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    Recently, there has been remarkable growth of interest in the development and applications of time-of-flight (ToF) depth cameras. Despite the permanent improvement of their characteristics, the practical applicability of ToF cameras is still limited by low resolution and quality of depth measurements. This has motivated many researchers to combine ToF cameras with other sensors in order to enhance and upsample depth images. In this paper, we review the approaches that couple ToF depth images with high-resolution optical images. Other classes of upsampling methods are also briefly discussed. Finally, we provide an overview of performance evaluation tests presented in the related studies

    Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow

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    At present, it is not clear how memory B lymphocytes are maintained over time, and whether only as circulating cells or also residing in particular tissues. Here we describe distinct populations of isotype-switched memory B lymphocytes (Bsm) of murine spleen and bone marrow, identified according to individual transcriptional signature and B cell receptor repertoire. A population of marginal zone-like cells is located exclusively in the spleen, while a population of quiescent Bsm is found only in the bone marrow. Three further resident populations, present in spleen and bone marrow, represent transitional and follicular B cells and B1 cells, respectively. A population representing 10-20% of spleen and bone marrow memory B cells is the only one qualifying as circulating. In the bone marrow, all cells individually dock onto VCAM1+ stromal cells and, reminiscent of resident memory T and plasma cells, are void of activation, proliferation and mobility

    Spin alignment and violation of the OZI rule in exclusive ω\omega and ϕ\phi production in pp collisions

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    Exclusive production of the isoscalar vector mesons ω\omega and ϕ\phi is measured with a 190 GeV/c/c proton beam impinging on a liquid hydrogen target. Cross section ratios are determined in three intervals of the Feynman variable xFx_{F} of the fast proton. A significant violation of the OZI rule is found, confirming earlier findings. Its kinematic dependence on xFx_{F} and on the invariant mass MpVM_{p\mathrm{V}} of the system formed by fast proton pfastp_\mathrm{fast} and vector meson VV is discussed in terms of diffractive production of pfastVp_\mathrm{fast}V resonances in competition with central production. The measurement of the spin density matrix element ρ00\rho_{00} of the vector mesons in different selected reference frames provides another handle to distinguish the contributions of these two major reaction types. Again, dependences of the alignment on xFx_{F} and on MpVM_{p\mathrm{V}} are found. Most of the observations can be traced back to the existence of several excited baryon states contributing to ω\omega production which are absent in the case of the ϕ\phi meson. Removing the low-mass MpVM_{p\mathrm{V}} resonant region, the OZI rule is found to be violated by a factor of eight, independently of xFx_\mathrm{F}.Comment: 23 pages, 13 figures and 5 table

    Multiplicities of charged pions and unidentified charged hadrons from deep-inelastic scattering of muons off an isoscalar target

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    Multiplicities of charged pions and unidentified hadrons produced in deep-inelastic scattering were measured in bins of the Bjorken scaling variable xx, the relative virtual-photon energy yy and the relative hadron energy zz. Data were obtained by the COMPASS Collaboration using a 160 GeV muon beam and an isoscalar target (6^6LiD). They cover the kinematic domain in the photon virtuality Q2Q^2 > 1(GeV/c)2)^2, 0.004<x<0.40.004 < x < 0.4, 0.2<z<0.850.2 < z < 0.85 and 0.1<y<0.70.1 < y < 0.7. In addition, a leading-order pQCD analysis was performed using the pion multiplicity results to extract quark fragmentation functions

    Non‐destructive automatic leaf area measurements by combining stereo and time‐of‐flight images

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    Leaf area measurements are commonly obtained by destructive and laborious practice. This study shows how stereo and time‐of‐flight (ToF) images can be combined for non‐destructive automatic leaf area measurements. The authors focus on some challenging plant images captured in a greenhouse environment, and show that even the state‐of‐the‐art stereo methods produce unsatisfactory results. By transforming depth information in a ToF image to a localised search range for dense stereo, a global optimisation strategy is adopted for producing smooth results that preserve discontinuity. They also use edges of colour and disparity images for automatic leaf detection and develop a smoothing method necessary for accurately estimating surface area. In addition to show that combining stereo and ToF images gives superior qualitative and quantitative results, 149 automatic measurements on leaf area using the authors system in a validation trial have a correlation of 0.97 with true values and the root‐mean‐square error is 10.97 cm2, which is 9.3% of the average leaf area. Their approach could potentially be applied for combining other modalities of images with large difference in image resolutions and camera positions

    Interplay among transversity induced asymmetries in hadron leptoproduction

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    In the fragmentation of a transversely polarized quark several left-right asymmetries are possible for the hadrons in the jet. When only one unpolarized hadron is selected, it exhibits an azimuthal modulation known as Collins effect. When a pair of oppositely charged hadrons is observed, three asymmetries can be considered, a di-hadron asymmetry and two single hadron asymmetries. In lepton deep inelastic scattering on transversely polarized nucleons all these asymmetries are coupled with the transversity distribution. From the high statistics COMPASS data on oppositely charged hadron-pair production we have investigated for the first time the dependence of these three asymmetries on the difference of the azimuthal angles of the two hadrons. The similarity of transversity induced single and di-hadron asymmetries is discussed. A new analysis of the data allows to establish quantitative relationships among them, providing for the first time strong experimental indication that the underlying fragmentation mechanisms are all driven by a common physical process.Comment: 6 figure

    Longitudinal double spin asymmetries in single hadron quasi-real photoproduction at high pTp_T

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    We measured the longitudinal double spin asymmetries ALLA_{LL} for single hadron muo-production off protons and deuterons at photon virtuality Q2Q^2 < 1(GeV/c\it c)2^2 for transverse hadron momenta pTp_T in the range 0.7 GeV/c\it c to 4 GeV/c\it c . They were determined using COMPASS data taken with a polarised muon beam of 160 GeV/c\it c or 200 GeV/c\it c impinging on polarised 6LiD\mathrm{{}^6LiD} or NH3\mathrm{NH_3} targets. The experimental asymmetries are compared to next-to-leading order pQCD calculations, and are sensitive to the gluon polarisation ΔG\Delta G inside the nucleon in the range of the nucleon momentum fraction carried by gluons 0.05<xg<0.20.05 < x_g < 0.2
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